Bentley opened the performance with a tribute to JT Gray, the owner of The Station Inn, who passed away in March.
Bentley previously recorded "Pride (In the Name of Love)," originally recorded by U2, for his 2010 album Up on the Ridge. The song, written about Martin Luther King Jr., was listed as one of Rolling Stone's "500 Greatest Songs of All Time."
Bentley told Entertainment Tonight he wanted to do something different for the new rendition of the tune.
"It takes on a whole new life when The War and Treaty came in. So, originally did the song with the Punch Brothers, [a] very cool bluegrass arrangement of a U2 song," Bentley said. "Obviously, I want to do something totally different, and, so it's great. Larkin Poe are these girls who play mandolin and over the song, Cocktails plays bass and fiddle. So we've got 3 rad girls shredding on this song."
The "Gone" singer said he believes the performance will be one of his proudest moments on stage.
"You have The War and Treaty singing all the big notes and taking the 3rd verse about Martin Luther King," Bentley said. "I dunno, it feels pretty, for me as much as I've done, this one feels like the one I'll be most proud of."
Bentley was nominated for Male Artist of the Year and Video of the Year for "Gone."
See the complete list of 2021 ACM Award winners here.